Using different promoter, driven by the green fluorescent protein gene transformation of tobacco in 。Obtain the rooting against kanamycin plant.The plant extract roots DNA,Using the PCR detection technology for testing,The exogenous gene has been determined,And the transformation of tobacco green fluorescent protein expression tissue specific analysis,Results show that:Transgenic tobacco in bright green fluorescent protein,35S promoters are expressed in any organization,And PNZIP promoters fails to express in roots,As the reference of general tobacco without conversion and fluorescence.Shows the different promoters green fluorescent protein expression with organization.