By reveed-phase HPLC cohosh puerarin particles of puerarin in content. Methods chromatographic conditions: chromatographic column: ф 4.6 x 250 mm using C18 column; Mobile phase: methanol-water (even 5); Detected wavelength: 250 nm. Flow rate: 1.0 ml/min; Into the sample weight: 20 μ l quantitative ring into the samples. Results in the chromatographic conditions, good linear relationship, reproducibility is 0.49%, the precision is 0.77%, and sample recycling the recovery rate is 0.76%. Conclusion cohosh puerarin grains contain pueraria puerarin in measuring not less than 3.00 mg/g.