With COX-2 anti-rat monoclonal antibody coated on the ELISA plate, and standard samples with the COX-2 monoclonal antibody, adding biotinylated anti-rat COX-2, the formation of immune complexes in connection board , Horseradish peroxidase labeled with Streptavidin biotin, adding at the end of the work-blue LCD, plus the final termination of the liquid sulfuric acid, in the Department 450nm measuring OD value, COX-2 concentration and OD value in direct proportion to the standard Commodities 20,10,5,2.5,1.25,0.625,0.312, 0 ng / ml for the abscissa, OD value of longitudinal coordinates, draw the standard curve, corresponding to identify COX-2 content. (Translated into English)