With COX-2 anti-rat monoclonal antibody coated on the ELISA plate, and standard samples with the COX-2 monoclonal antibody, adding biotinylated anti-rat COX-2, the formation of immune complexes in connection board , Horseradish peroxidase labeled with Streptavidin biotin, adding at the end of the work-blue LCD, plus the final termination of the liquid sulfuric acid, in the Department 450nm measuring OD value, COX-2 concentration and OD value in direct proportion to the standard Commodities 20,10,5,2.5,1.25,0.625,0.312, 0 ng / ml for the abscissa, OD value of longitudinal coordinates, draw the standard curve, corresponding to identify COX-2 content
With COX-2 anti-rat monoclonal antibody coated on the ELISA plate, and standard samples with the COX-2 monoclonal antibody, adding biotinylated anti-rat COX-2, the formation of immune complexes in connection board , Horseradish peroxidase labeled with Streptavidin biotin, adding at the end of the work-blue LCD, plus the final termination of the liquid sulfuric acid, in the Department 450nm measuring OD value, COX-2 concentration and OD value in direct proportion to the standard Commodities 20,10,5,2.5,1.25,0.625,0.312, 0 ng / ml for the abscissa, OD value of longitudinal coordinates, draw the standard curve, corresponding to identify COX-2 content. (Translated into English)
教你一个简单的方法参考同类文献的摘要翻译方法。还有可以参考外文的写法。另外可以使用google的翻译功能,不过需要重新整理。google的翻译转录如下(你自己整理吧)Objective: To investigate the serum protein electrophoresis in patients with liver cirrhosis spectrum Change for clinical diagnosis and treatment of the provision of reference materials. Methods: The United States Helena SPIFE 2000 agarose electrophoresis Automatic Determination of 138 cases and 50 cases of liver cirrhosis in patients with normal serum protein ratio. Results: The patients with liver cirrhosis of serum protein electrophoresis of each component compared with the normal result, there is significant difference. Conclusion: in patients with cirrhosis of the serum protein electrophoresis analysis must have the characteristics of cirrhosis are the diagnosis, prognosis and efficacy of a good indicator.