[Abstract]: Objective: To make use of electric spinning method for preparation of E. coli phage template molecular imprinting polymer film. Requirements in this experiment are familiar with the whole process of scientific research, including data collection, the subject design, the experimental operation and the writing of professional papers. At the same time improve the thinking ability of students to scientific research and practical hands-capacity.Methods: PEG precipitation + chloroform extraction purification of phage enrichment after reaching 1012/ml, by adjusting the concentration of PVA, flow, voltage, and other conditions of the high spinning, film prepared by scanning electron microscopy (SEM) observation of spin silk solution concentration, flow rate, voltage, and other high fiber spinning conditions on morphology and diameter of the impact. Electrospinning technology use in the above conditions and explore the preparation of a PVA for the "shell", E. coli phage as a "core" of the molecularly imprinted membrane, and through observation of double-layer agar method Plaque packaged phage detection of biological activity.Results: The success of purification of phage concentration to achieve the above 1012/ml. Groping in the process of the preparation conditions with water as the solvent for the PVA concentration of 7% (w / w) of the membrane 2; in the SM by adding ethanol and, after spinning a very unstable and a large number of broken wires, beads, drip phenomenon, not film, due to time reasons, did not continue, the resulting termination of the experiment. Turn to the morphology of PVA fiber spinning, the diameter, such as PVA concentration, exploring the relationship between voltageCONCLUSION: ① prepared by the observation of a few films The results showed that the concentration of spinning solution with the increase in fiber diameter increases, the distribution of fiber diameter increases; as the voltage increases, fiber diameter did not change significantly.② experiment to make adequate preparations, phage with no spinning PVA polymer matrix to assess the compatibility.[Key words]: phage titer PVA electrospinning